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All procedures were approved by the Animal Ethics Committee (AEC) of The University of Western Australia (RA/3/100/1613), with reciprocal approval from the AEC of Curtin University (ARE2019-4), and were performed in accordance with the principles of the National Health and Medical Research Council of Australia
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Transcriptional factors (OCT4, SOX2, and NANOG) form a core regulatory hub in hESCs, interacting with each other and co-occupying their regulatory regions to create autoregulatory loops that stabilize their expression 7,8